Preliminary research on tissue culture of Chinese sturgeon

Ye XH, Liu HQ, Yu XM, Zhang YB, Chang J B

Cell strains were established from snout and gonad tissues of Chinese sturgeon. Acipenser sinensis, among eight tissues for primary culture and designated CSSn and CSG, respectively. Biological characteristics were partly examined for CSSn and CSG. Both strains appeared fibroblastic. They had large quantities of chromosomes and possessed microchromosomes. The distributions of their chromosome number exhibited the same feature described as two wave crests distinguished and a great variety of distribution, which demonstrated that they became aneuploid. Growth curve of both strains showed their relatively slow replication. The highest cell number of CSG was observed after incubated 9 days, while CSSn, 14 days. The effects of temperature and PH on growth were examined for CSG, CSG remained a normal state at 16 - 30°C , with optimum temperature of 27°C, and pH condition of 7.2.